Cell cycle regulation of the cyclin A gene promoter is mediated by a variant E2F site.
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Cyclin A is involved in the control of S phase and mitosis in mammalian cells. Expression of the cyclin A gene in nontransformed cells is characterized by repression of its promoter during the G1 phase of the cell cycle and its induction at S-phase entry. We show that this mode of regulation is mediated by the transcription factor E2F, which binds to a specific site in the cyclin A promoter. It differs from the prototype E2F site in nucleotide sequence and protein binding; it is bound by E2F complexes containing cyclin E and p107 but not pRB. Ectopic expression of cyclin D1 triggers premature activation of the cyclin A promoter by E2F, and this effect is blocked by the tumor suppressor protein p16INK4.
Original language | English |
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Journal | Proceedings of the National Academy of Science of the United States of America |
Volume | 92 |
Issue number | 24 |
Pages (from-to) | 11264-8 |
Number of pages | 4 |
ISSN | 0027-8424 |
Publication status | Published - 1995 |
Bibliographical note
Keywords: 3T3 Cells; Animals; Base Sequence; Carrier Proteins; Cell Cycle; Cell Cycle Proteins; Cyclin D1; Cyclin-Dependent Kinase 4; Cyclin-Dependent Kinase Inhibitor p16; Cyclin-Dependent Kinases; Cyclins; DNA-Binding Proteins; E2F Transcription Factors; Gene Expression; Mice; Molecular Sequence Data; Oligodeoxyribonucleotides; Oncogene Proteins; Promoter Regions (Genetics); Proto-Oncogene Proteins; RNA, Messenger; Trans-Activation (Genetics); Transcription Factor DP1; Transcription Factors; Transcription, Genetic
ID: 5053151