Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase

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Standard

Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase. / Krath, Britta N.; Hove-Jensen, Bjarne.

In: Gene, Vol. 176, No. 1-2, 1996, p. 73-79.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Krath, BN & Hove-Jensen, B 1996, 'Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase', Gene, vol. 176, no. 1-2, pp. 73-79. https://doi.org/10.1016/0378-1119(96)00222-3

APA

Krath, B. N., & Hove-Jensen, B. (1996). Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase. Gene, 176(1-2), 73-79. https://doi.org/10.1016/0378-1119(96)00222-3

Vancouver

Krath BN, Hove-Jensen B. Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase. Gene. 1996;176(1-2):73-79. https://doi.org/10.1016/0378-1119(96)00222-3

Author

Krath, Britta N. ; Hove-Jensen, Bjarne. / Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase. In: Gene. 1996 ; Vol. 176, No. 1-2. pp. 73-79.

Bibtex

@article{70152fa0cea311dcbee902004c4f4f50,
title = "Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase",
abstract = "The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-l-phosphate uridyltransferase upstream of prs, and a gene homologous to ctc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis",
author = "Krath, {Britta N.} and Bjarne Hove-Jensen",
note = "Author Keywords: N-Acetylglucosamine-l-phosphate uridyltransferase; cDNA; ctc; gcaD; Operon expression; Phosphoribosylpyrophosphate; Phosphoribosyl diphosphate; Thermophile; tms",
year = "1996",
doi = "10.1016/0378-1119(96)00222-3",
language = "English",
volume = "176",
pages = "73--79",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Bacillus caldolyticus prs gene encoding phosphoribosyldiphosphate synthase

AU - Krath, Britta N.

AU - Hove-Jensen, Bjarne

N1 - Author Keywords: N-Acetylglucosamine-l-phosphate uridyltransferase; cDNA; ctc; gcaD; Operon expression; Phosphoribosylpyrophosphate; Phosphoribosyl diphosphate; Thermophile; tms

PY - 1996

Y1 - 1996

N2 - The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-l-phosphate uridyltransferase upstream of prs, and a gene homologous to ctc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis

AB - The prs gene, encoding phosphoribosyl-diphosphate (PRPP) synthase, as well as the flanking DNA sequences were cloned and sequenced from the Gram-positive thermophile, Bacillus caldolyticus. Comparison with the homologous sequences from the mesophile, Bacillus subtilis, revealed a gene (gcaD) encoding N-acetylglucosamine-l-phosphate uridyltransferase upstream of prs, and a gene homologous to ctc downstream of prs. cDNA synthesis with a B. caldolyticus gcaD-prs-ctc-specified mRNA as template, followed by amplification utilising the polymerase chain reaction indicated that the three genes are co-transcribed. Comparison of amino acid sequences revealed a high similarity among PRPP synthases across a wide phylogenetic range. An E. coli strain harbouring the B. caldolyticus prs gene in a multicopy plasmid produced PRPP synthase activity 33-fold over the activity of a haploid B. caldolyticus strain. B. caldolyticus PRPP synthase was resistant to heat treatment at 70°C to a much higher extent than PRPP synthase from B. subtilis

U2 - 10.1016/0378-1119(96)00222-3

DO - 10.1016/0378-1119(96)00222-3

M3 - Journal article

VL - 176

SP - 73

EP - 79

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -

ID: 2508365