A short-term in vitro test for tumour sensitivity to adriamycin based on flow cytometric DNA analysis
Research output: Contribution to journal › Journal article › Research › peer-review
A new method to test the sensitivity of tumour cells to chemotherapy is presented. Tumour cells were incubated in vitro on agar, and drug-induced cell cycle perturbation was monitored by flow cytometric DNA analysis. In the present study the method was applied to monitor the effect of adriamycin on an adriamycin-sensitive Ehrlich ascites tumour and two adriamycin-resistant tumours. Adriamycin caused a dose-related accumulation of tumour cells in the G2 + M phase in the sensitive tumour. Drug concentrations greater than or equal to 100-fold higher were required to induce similar changes in the resistant tumours. The dose level causing maximum accumulation in the G2 + M phase is suggested as a parameter for quantifying the sensitivity. The results indicate that the method can be extended to sensitivity testing of human tumours.
Original language | English |
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Journal | British Journal of Cancer |
Volume | 47 |
Issue number | 4 |
Pages (from-to) | 497-502 |
Number of pages | 5 |
ISSN | 0007-0920 |
Publication status | Published - 1983 |
Bibliographical note
Keywords: Animals; Carcinoma, Ehrlich Tumor; Cell Cycle; Cells, Cultured; DNA, Neoplasm; Dose-Response Relationship, Drug; Doxorubicin; Drug Resistance; Flow Cytometry; Mice
ID: 12872558