Uptake of benzimidazoles by Trichuris suis in vivo in pigs

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Uptake of benzimidazoles by Trichuris suis in vivo in pigs. / Hansen, Tina Vicky Alstrup; Friis, Christian; Nejsum, Peter; Olsen, Annette; Thamsborg, Stig Milan.

In: International Journal for Parasitology: Drugs and Drug Resistance, Vol. 4, No. 2, 2014, p. 112-117.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Hansen, TVA, Friis, C, Nejsum, P, Olsen, A & Thamsborg, SM 2014, 'Uptake of benzimidazoles by Trichuris suis in vivo in pigs', International Journal for Parasitology: Drugs and Drug Resistance, vol. 4, no. 2, pp. 112-117. https://doi.org/10.1016/j.ijpddr.2014.03.003

APA

Hansen, T. V. A., Friis, C., Nejsum, P., Olsen, A., & Thamsborg, S. M. (2014). Uptake of benzimidazoles by Trichuris suis in vivo in pigs. International Journal for Parasitology: Drugs and Drug Resistance, 4(2), 112-117. https://doi.org/10.1016/j.ijpddr.2014.03.003

Vancouver

Hansen TVA, Friis C, Nejsum P, Olsen A, Thamsborg SM. Uptake of benzimidazoles by Trichuris suis in vivo in pigs. International Journal for Parasitology: Drugs and Drug Resistance. 2014;4(2):112-117. https://doi.org/10.1016/j.ijpddr.2014.03.003

Author

Hansen, Tina Vicky Alstrup ; Friis, Christian ; Nejsum, Peter ; Olsen, Annette ; Thamsborg, Stig Milan. / Uptake of benzimidazoles by Trichuris suis in vivo in pigs. In: International Journal for Parasitology: Drugs and Drug Resistance. 2014 ; Vol. 4, No. 2. pp. 112-117.

Bibtex

@article{7348b0498eaa4616819e5b2ef407c8b4,
title = "Uptake of benzimidazoles by Trichuris suis in vivo in pigs",
abstract = "It is recognized that the clinical efficacy of single dose benzimidazoles (BZs) against the nematode, Trichuris suis of pigs and the closely related Trichuris trichiura in humans is only poor to moderate. Recent in vitro studies have indicated that a low uptake of fenbendazole (FBZ) in T. suis may be responsible for its poor efficacy. The aim of this study was to investigate this hypothesis by measuring the concentrations of FBZ and its metabolites, oxfendazole (OXF) and FBZ sulphone (FBZSO2), in T. suis isolated from FBZ treated pigs and in plasma of the pigs. The highest concentration of FBZ measured in T. suis was 66.6 pmol/mg dry worm tissue which was approximately half of what was measured in a previous in vitro study. The correlation between drug concentrations in plasma and in T. suis worms was highly positive for OXF (r = 0.93, P = 0.0007) and FBZSO2 (r = 0.85, P = 0.007), but no correlation was found for FBZ. This study shows that the low uptake of FBZ observed for T. suis in vitro, also takes place in vivo. The high and significant correlations between OXF and FBZSO2 concentrations in plasma of the pigs and T. suis (and the lack of this correlation for FBZ) suggests that the metabolites reach the worms via the blood-enterocyte interface while FBZ primarily reaches the worms via the intestinal lumen of the host.",
keywords = "Faculty of Health and Medical Sciences, Trichuris trichiura, Trichuris suis, Benzimidazole, Drug-uptake, Treatment efficacy",
author = "Hansen, {Tina Vicky Alstrup} and Christian Friis and Peter Nejsum and Annette Olsen and Thamsborg, {Stig Milan}",
year = "2014",
doi = "10.1016/j.ijpddr.2014.03.003",
language = "English",
volume = "4",
pages = "112--117",
journal = "International Journal for Parasitology: Drugs and Drug Resistance",
issn = "2211-3207",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Uptake of benzimidazoles by Trichuris suis in vivo in pigs

AU - Hansen, Tina Vicky Alstrup

AU - Friis, Christian

AU - Nejsum, Peter

AU - Olsen, Annette

AU - Thamsborg, Stig Milan

PY - 2014

Y1 - 2014

N2 - It is recognized that the clinical efficacy of single dose benzimidazoles (BZs) against the nematode, Trichuris suis of pigs and the closely related Trichuris trichiura in humans is only poor to moderate. Recent in vitro studies have indicated that a low uptake of fenbendazole (FBZ) in T. suis may be responsible for its poor efficacy. The aim of this study was to investigate this hypothesis by measuring the concentrations of FBZ and its metabolites, oxfendazole (OXF) and FBZ sulphone (FBZSO2), in T. suis isolated from FBZ treated pigs and in plasma of the pigs. The highest concentration of FBZ measured in T. suis was 66.6 pmol/mg dry worm tissue which was approximately half of what was measured in a previous in vitro study. The correlation between drug concentrations in plasma and in T. suis worms was highly positive for OXF (r = 0.93, P = 0.0007) and FBZSO2 (r = 0.85, P = 0.007), but no correlation was found for FBZ. This study shows that the low uptake of FBZ observed for T. suis in vitro, also takes place in vivo. The high and significant correlations between OXF and FBZSO2 concentrations in plasma of the pigs and T. suis (and the lack of this correlation for FBZ) suggests that the metabolites reach the worms via the blood-enterocyte interface while FBZ primarily reaches the worms via the intestinal lumen of the host.

AB - It is recognized that the clinical efficacy of single dose benzimidazoles (BZs) against the nematode, Trichuris suis of pigs and the closely related Trichuris trichiura in humans is only poor to moderate. Recent in vitro studies have indicated that a low uptake of fenbendazole (FBZ) in T. suis may be responsible for its poor efficacy. The aim of this study was to investigate this hypothesis by measuring the concentrations of FBZ and its metabolites, oxfendazole (OXF) and FBZ sulphone (FBZSO2), in T. suis isolated from FBZ treated pigs and in plasma of the pigs. The highest concentration of FBZ measured in T. suis was 66.6 pmol/mg dry worm tissue which was approximately half of what was measured in a previous in vitro study. The correlation between drug concentrations in plasma and in T. suis worms was highly positive for OXF (r = 0.93, P = 0.0007) and FBZSO2 (r = 0.85, P = 0.007), but no correlation was found for FBZ. This study shows that the low uptake of FBZ observed for T. suis in vitro, also takes place in vivo. The high and significant correlations between OXF and FBZSO2 concentrations in plasma of the pigs and T. suis (and the lack of this correlation for FBZ) suggests that the metabolites reach the worms via the blood-enterocyte interface while FBZ primarily reaches the worms via the intestinal lumen of the host.

KW - Faculty of Health and Medical Sciences

KW - Trichuris trichiura

KW - Trichuris suis

KW - Benzimidazole

KW - Drug-uptake

KW - Treatment efficacy

U2 - 10.1016/j.ijpddr.2014.03.003

DO - 10.1016/j.ijpddr.2014.03.003

M3 - Journal article

C2 - 25057460

VL - 4

SP - 112

EP - 117

JO - International Journal for Parasitology: Drugs and Drug Resistance

JF - International Journal for Parasitology: Drugs and Drug Resistance

SN - 2211-3207

IS - 2

ER -

ID: 122666894