Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos. / Find, Jens Iver; Hargreaves, Cathy L.; Reeves, Catherine B. .

In: In Vitro Cellular & Developmental Biology - Plant, Vol. 50, No. 2, 2014, p. 190-198.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Find, JI, Hargreaves, CL & Reeves, CB 2014, 'Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos', In Vitro Cellular & Developmental Biology - Plant, vol. 50, no. 2, pp. 190-198. https://doi.org/10.1007/s11627-013-9581-1

APA

Find, J. I., Hargreaves, C. L., & Reeves, C. B. (2014). Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos. In Vitro Cellular & Developmental Biology - Plant, 50(2), 190-198. https://doi.org/10.1007/s11627-013-9581-1

Vancouver

Find JI, Hargreaves CL, Reeves CB. Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos. In Vitro Cellular & Developmental Biology - Plant. 2014;50(2):190-198. https://doi.org/10.1007/s11627-013-9581-1

Author

Find, Jens Iver ; Hargreaves, Cathy L. ; Reeves, Catherine B. . / Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos. In: In Vitro Cellular & Developmental Biology - Plant. 2014 ; Vol. 50, No. 2. pp. 190-198.

Bibtex

@article{ba9c85c9310d43f3a15d9a66f51323c2,
title = "Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos",
abstract = "Green cones of radiata pine were collected from two open-pollinated, elite families in two successive years at weekly intervals, and initiation of embryogenic cultures was investigated as a function of sampling date, initiation medium, explant type, and developmental stage. A combination of dissected embryos and a modified Litvay medium, Glitz, was best. This combination gave the highest rate of initiation, and it was possible to initiate somatic embryogenesis (SE) from differentiated cells in the epicotyledonary region of postcotyledonary zygotic embryos from the two tested families with an average initiation rate of approximately 24% and 7% from stage five and six embryos, respectively. This is different from established initiation protocols of embryogenic cultures in radiata pine, which has traditionally been based on embryo rescue and continued proliferation of immature zygotic embryos. A further implication of initiation of SE from excised post-cotyledonary embryos was that the period of initiation of embryogenic cultures was extended from 4 to 12 wk. ",
keywords = "???Bioteknologi og planter???, Somatic embryogenesis . Radiata pine . Initiation . Conifer",
author = "Find, {Jens Iver} and Hargreaves, {Cathy L.} and Reeves, {Catherine B.}",
year = "2014",
doi = "10.1007/s11627-013-9581-1",
language = "English",
volume = "50",
pages = "190--198",
journal = "In Vitro",
issn = "1054-5476",
publisher = "Springer",
number = "2",

}

RIS

TY - JOUR

T1 - Progress towards initiation of somatic embryogenesis from differentiated tissues of radiata pine (Pinus radiata D. Don) using cotyledonary embryos

AU - Find, Jens Iver

AU - Hargreaves, Cathy L.

AU - Reeves, Catherine B.

PY - 2014

Y1 - 2014

N2 - Green cones of radiata pine were collected from two open-pollinated, elite families in two successive years at weekly intervals, and initiation of embryogenic cultures was investigated as a function of sampling date, initiation medium, explant type, and developmental stage. A combination of dissected embryos and a modified Litvay medium, Glitz, was best. This combination gave the highest rate of initiation, and it was possible to initiate somatic embryogenesis (SE) from differentiated cells in the epicotyledonary region of postcotyledonary zygotic embryos from the two tested families with an average initiation rate of approximately 24% and 7% from stage five and six embryos, respectively. This is different from established initiation protocols of embryogenic cultures in radiata pine, which has traditionally been based on embryo rescue and continued proliferation of immature zygotic embryos. A further implication of initiation of SE from excised post-cotyledonary embryos was that the period of initiation of embryogenic cultures was extended from 4 to 12 wk.

AB - Green cones of radiata pine were collected from two open-pollinated, elite families in two successive years at weekly intervals, and initiation of embryogenic cultures was investigated as a function of sampling date, initiation medium, explant type, and developmental stage. A combination of dissected embryos and a modified Litvay medium, Glitz, was best. This combination gave the highest rate of initiation, and it was possible to initiate somatic embryogenesis (SE) from differentiated cells in the epicotyledonary region of postcotyledonary zygotic embryos from the two tested families with an average initiation rate of approximately 24% and 7% from stage five and six embryos, respectively. This is different from established initiation protocols of embryogenic cultures in radiata pine, which has traditionally been based on embryo rescue and continued proliferation of immature zygotic embryos. A further implication of initiation of SE from excised post-cotyledonary embryos was that the period of initiation of embryogenic cultures was extended from 4 to 12 wk.

KW - ???Bioteknologi og planter???

KW - Somatic embryogenesis . Radiata pine . Initiation . Conifer

U2 - 10.1007/s11627-013-9581-1

DO - 10.1007/s11627-013-9581-1

M3 - Journal article

VL - 50

SP - 190

EP - 198

JO - In Vitro

JF - In Vitro

SN - 1054-5476

IS - 2

ER -

ID: 96238948