Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

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Standard

Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). / Jensen, Lars Jørn; Salomonsson, Max; Sørensen, Charlotte Mehlin; von Holstein-Rathlou, Niels-Henrik; Pedersen, Henrik Duelund; Christoffersen, Berit Ø.; Olsen, Lisbeth Høier; Ludvigsen, Trine Pagh.

In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 28, No. Supplement 1, 676.4, 27.04.2014.

Research output: Contribution to journalConference abstract in journalResearchpeer-review

Harvard

Jensen, LJ, Salomonsson, M, Sørensen, CM, von Holstein-Rathlou, N-H, Pedersen, HD, Christoffersen, BØ, Olsen, LH & Ludvigsen, TP 2014, 'Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, vol. 28, no. Supplement 1, 676.4. https://doi.org/10.1096/fj.1530-6860

APA

Jensen, L. J., Salomonsson, M., Sørensen, C. M., von Holstein-Rathlou, N-H., Pedersen, H. D., Christoffersen, B. Ø., ... Ludvigsen, T. P. (2014). Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 28(Supplement 1), [676.4]. https://doi.org/10.1096/fj.1530-6860

Vancouver

Jensen LJ, Salomonsson M, Sørensen CM, von Holstein-Rathlou N-H, Pedersen HD, Christoffersen BØ et al. Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Apr 27;28(Supplement 1). 676.4. https://doi.org/10.1096/fj.1530-6860

Author

Jensen, Lars Jørn ; Salomonsson, Max ; Sørensen, Charlotte Mehlin ; von Holstein-Rathlou, Niels-Henrik ; Pedersen, Henrik Duelund ; Christoffersen, Berit Ø. ; Olsen, Lisbeth Høier ; Ludvigsen, Trine Pagh. / Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster). In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 ; Vol. 28, No. Supplement 1.

Bibtex

@article{7f38bc0335924d9d9c4bb8b3458bd492,
title = "Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)",
abstract = "Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male G{\"o}ttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.",
keywords = "Faculty of Health and Medical Sciences, Obesity, Arteries, Potassium Channels, Calcium Channels, Gene expression, metabolic syndrome, Rat, Pig",
author = "Jensen, {Lars J{\o}rn} and Max Salomonsson and S{\o}rensen, {Charlotte Mehlin} and {von Holstein-Rathlou}, Niels-Henrik and Pedersen, {Henrik Duelund} and Christoffersen, {Berit {\O}.} and Olsen, {Lisbeth H{\o}ier} and Ludvigsen, {Trine Pagh}",
note = "Faseb Journal, Vol. 28, No. 1 Suppl. 676.4",
year = "2014",
month = "4",
day = "27",
doi = "10.1096/fj.1530-6860",
language = "English",
volume = "28",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "Supplement 1",

}

RIS

TY - ABST

T1 - Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

AU - Jensen, Lars Jørn

AU - Salomonsson, Max

AU - Sørensen, Charlotte Mehlin

AU - von Holstein-Rathlou, Niels-Henrik

AU - Pedersen, Henrik Duelund

AU - Christoffersen, Berit Ø.

AU - Olsen, Lisbeth Høier

AU - Ludvigsen, Trine Pagh

N1 - Faseb Journal, Vol. 28, No. 1 Suppl. 676.4

PY - 2014/4/27

Y1 - 2014/4/27

N2 - Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.

AB - Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.

KW - Faculty of Health and Medical Sciences

KW - Obesity

KW - Arteries

KW - Potassium Channels

KW - Calcium Channels

KW - Gene expression

KW - metabolic syndrome

KW - Rat

KW - Pig

U2 - 10.1096/fj.1530-6860

DO - 10.1096/fj.1530-6860

M3 - Conference abstract in journal

VL - 28

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - Supplement 1

M1 - 676.4

ER -

ID: 139851558