Discovery of a bacterial gene cluster for deglycosylation of the toxic potato steroidal glycoalkaloids α-chaconine and α-solanine
Research output: Contribution to journal › Journal article › peer-review
Potato juice is a by-product of starch processing currently used as feed. However, potato proteins are an untapped source of high-protein food for human nutrition if harmful constituents notably glycoalkaloids (GAs) are detoxified. The two principle GAs found in potato are α-chaconine and α-solanine, both consisting of a solanidine aglycone with a carbohydrate side-chain. The first step in the detoxification of these compounds is removal of the trisaccharide. Whole-genome sequencing of a bacterial isolate, Arthrobacter sp. S41, that degrade completely α-chaconine and α-solanine revealed the presence of a gene cluster possibly involved in the deglycosylation of GAs. Functional characterization confirmed the enzymatic activity of the gene cluster involved in the complete deglycosylation of both α-chaconine and α-solanine. The novel enzymes described here may find value in the bioconversion of feed proteins to food proteins suitable for human nutrition.
Original language | English |
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Journal | Journal of Agricultural and Food Chemistry |
Volume | 68 |
Issue number | 5 |
Pages (from-to) | 1390-1396 |
Number of pages | 7 |
ISSN | 0021-8561 |
DOIs | |
Publication status | Published - 2020 |
ID: 234346842