Cytokine stimulation of the choriocarcinoma cell line JEG-3 leads to alterations in the HLA-G expression profile

Research output: Contribution to journalJournal articleResearchpeer-review

  • Gry Persson
  • Julie Birgit Siig Bork
  • Cecilie Isgaard
  • Tine Graakjær Larsen
  • Anna Maria Bordoy
  • Meghan Sand Bengtsson
  • Hviid, Thomas Vauvert F.

The checkpoint molecule human leukocyte antigen (HLA)-G has restricted tissue expression, and plays a role in the establishment of maternal tolerance to the semi-allogenic fetus during pregnancy by expression on the trophoblast cells in the placenta. HLA-G exists in at least seven well-described mRNA isoforms, of which four are membrane-bound and three soluble. Regulation of the tissue expression of HLA-G and its isoforms is relatively unknown. Therefore, it is important to understand the regulation of HLA-G, and the HLA-G+ choriocarcinoma cell line JEG-3 is a widely used cellular model. We hypothesized that cytokines present in the microenvironment can regulate the HLA-G expression profile. In the present study, we systematically stimulated JEG-3 cells with various concentrations of IL-2, IL-4 IL-6, IL-10, IL-12, IL-15, IL-17A, TGF-β1, TNF-α and IFN-γ1b. The results suggest that IFN-γ plays a role in maintenance of HLA-G expression, while IL-10 might be involved in regulation of the isoform profile.

Original languageEnglish
JournalCellular Immunology
Volume352
Pages (from-to)104110
ISSN0008-8749
DOIs
Publication statusPublished - Jun 2020

ID: 241216135