Crx broadly modulates the pineal transcriptome
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Crx broadly modulates the pineal transcriptome. / Rovsing, Louise; Clokie, Samuel; Bustos, Diego M; Rohde, Kristian; Coon, Steven L; Litman, Thomas; Rath, Martin F; Møller, Morten; Klein, David C.
In: Journal of Neurochemistry, Vol. 119, No. 2, 2011, p. 262-74.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Crx broadly modulates the pineal transcriptome
AU - Rovsing, Louise
AU - Clokie, Samuel
AU - Bustos, Diego M
AU - Rohde, Kristian
AU - Coon, Steven L
AU - Litman, Thomas
AU - Rath, Martin F
AU - Møller, Morten
AU - Klein, David C
N1 - Journal of Neurochemistry © 2011 International Society for Neurochemistry.
PY - 2011
Y1 - 2011
N2 - Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa et al. 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx-/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
AB - Cone-rod homeobox (Crx) encodes Crx, a transcription factor expressed selectively in retinal photoreceptors and pinealocytes, the major cell type of the pineal gland. In this study, the influence of Crx on the mammalian pineal gland was studied by light and electron microscopy and by use of microarray and qRTPCR technology, thereby extending previous studies on selected genes (Furukawa et al. 1999). Deletion of Crx was not found to alter pineal morphology, but was found to broadly modulate the mouse pineal transcriptome, characterized by a > 2-fold down-regulation of 543 genes and a > 2-fold up-regulation of 745 genes (p < 0.05). Of these, one of the most highly up-regulated (18-fold) was Hoxc4, a member of the Hox gene family, members of which are known to control gene expression cascades. During a 24-h period, a set of 51 genes exhibited differential day/night expression in pineal glands of wild-type animals; only eight of these were also day/night expressed in the Crx-/- pineal gland. However, in the Crx-/- pineal gland 41 genes exhibited differential night/day expression that was not seen in wild-type animals. These findings indicate that Crx broadly modulates the pineal transcriptome and also influences differential night/day gene expression in this tissue. Some effects of Crx deletion on the pineal transcriptome might be mediated by Hoxc4 up-regulation.
KW - Faculty of Health and Medical Sciences
KW - neurobiology, circadian rhythms, gene expression
U2 - 10.1111/j.1471-4159.2011.07405.x
DO - 10.1111/j.1471-4159.2011.07405.x
M3 - Journal article
C2 - 21797868
VL - 119
SP - 262
EP - 274
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 2
ER -
ID: 33855154