Nicholas M I Taylor

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Several complex biological molecular machineries exist that transfer molecules across lipid bilayers. In the group, we use a combination of cryo-electron microscopy and functional techniques to investigate the structure and mechanism of these systems. Of special interest to us are secretion systems of bacteria, which transfer protein molecules across the lipid bilayer, and contractile injection systems related to contractile bacteriophages which inject DNA and proteins into the cell. We also investigate human membrane transport proteins involved in health and disease.

Our overarching goal is to elucidate how transport across the membrane is achieved as well as regulated. We aim to uncover shared principles as well as fundamental differences between membrane transport systems and are especially interested in investigating these machineries in a near-native, lipid bilayer environment. Apart from gaining fundamental insight into transport across the membrane, our results can also aid structure-based drug design.

Selected publications

1. Published

   Cryo-EM structures of a human ABCG2 mutant trapped in ATP-bound and substrate-bound states

   Manolaridis, I., Jackson, S. M., Taylor, Nicholas M I, Kowal, J., Stahlberg, H. & Locher, K. P., 2018, In: Nature. 563, 7731, p. 426-430 5 p.

   Research output: Contribution to journal › Letter › Research › peer-review

2. Structure of the human multidrug transporter ABCG2

   Taylor, Nicholas M I, Manolaridis, I., Jackson, S. M., Kowal, J., Stahlberg, H. & Locher, K. P., 22 Jun 2017, In: Nature. 546, 7659, p. 504-509 6 p.

   Research output: Contribution to journal › Journal article › Research › peer-review

3. Structure of the T4 baseplate and its function in triggering sheath contraction

   Taylor, Nicholas M I, Prokhorov, N. S., Guerrero-Ferreira, R. C., Shneider, M. M., Browning, C., Goldie, K. N., Stahlberg, H. & Leiman, P. G., 2016, In: Nature. 533, 7603, p. 346-352 7 p.

   Research output: Contribution to journal › Journal article › Research › peer-review

4. Crystal structure of the 14-subunit RNA polymerase I

   Fernández-Tornero, C., Moreno-Morcillo, M., Rashid, U. J., Taylor, Nicholas M I, Ruiz, F. M., Gruene, T., Legrand, P., Steuerwald, U. & Müller, C. W., 2013, In: Nature. 502, 7473, p. 644-9 6 p.

   Research output: Contribution to journal › Journal article › Research › peer-review

5. RNA polymerase III-specific general transcription factor IIIC contains a heterodimer resembling TFIIF Rap30/Rap74

   Taylor, Nicholas M I, Baudin, F., von Scheven, G. & Müller, C. W., 2013, In: Nucleic Acids Research. 41, 19, p. 9183-9196 14 p.

   Research output: Contribution to journal › Journal article › Research › peer-review

6. Structural and functional characterization of a phosphatase domain within yeast general transcription factor IIIC

   Taylor, Nicholas M I, Glatt, S., Hennrich, M. L., von Scheven, G., Grötsch, H., Fernández-Tornero, C., Rybin, V., Gavin, A., Kolb, P. & Müller, C. W., 2013, In: Journal of Biological Chemistry. 288, 21, p. 15110-15120 11 p.

   Research output: Contribution to journal › Journal article › Research › peer-review