Purification, characterization and ELISA detection of mink immunoglobulins

Research output: Contribution to journalJournal articlepeer-review

  • Cyril Jean-Marie Martel
  • Bent Aasted
This study describes easy purification methods for mink IgG, IgA and IgM immunoglobulins. IgG and IgM were purified from normal mink serum, while IgA was purified from mink bile from healthy animals. By SDS-polyacrylamid-gel-electrophoresis  (SDS-PAGE) and immunoblotting under reducing conditions the estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa respectively. The purities of purified IgG, IgM and IgA were estimated by immunoglobulin class specific ELISAs to be more than 90% for IgG and IgM, and more than 80% for IgA.
Original languageEnglish
JournalScientifur
Volume32
Issue number1
Pages (from-to)33-40
Number of pages8
ISSN0105-2403
Publication statusPublished - 2008

ID: 8297148